DMEM, High Glucose, GlutaPlus, Sodium Pyruvate, no HEPES. This product is used in the culture of cells with fast growth and low adhesion, and a variety of mammalian primary cells. Dulbecco’s Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors.Nneed to add 5-10% serum or serum-free additives depending on the cell type.This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 4.0 mM L-alanyl-L-glutamine and 1.0 mM sodium pyruvate, phenol red indicator,no HEPES buffer system.L-alanyl-L-glutamine, is a cell culture additive that replaces medium moles of L-glutamine in cell culture media. The substance is stable in aqueous solution and does not degrade spontaneously. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is thenabsorbed and used by cells.